SIMULTANEOUS DETECTION OF 14 ITALIAN CYSTIC-FIBROSIS MUTATIONS IN 7 EXONS BY REVERSE DOT-BLOT ANALYSIS

The increasing number of cystic fibrosis (CF) mutations is a great obs tacle to the use of DNA procedures in the detection of gene defects. W e describe a fast, cheap and non-radioactive procedure, Reverse dot-bl ot analysis (RDB), for the simultaneous detection of CF mutations in t he Italian population. We used this approach to study seven exons of t he CF gene for 14 CF gene defects and were able to characterize 222 of 272 CF chromosomes (80%). The cost of the procedure was $25 per sampl e analysed. (C) 1995 Academic Press Limited