ADDITIONAL CRYSTAL FORMS OF THE ESCHERICHIA-COLI CLASS-II FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE

We have obtained two additional crystal forms of the metal-dependent c lass II fructose-1,6-bisphosphate aldolase from Escherichia coil. Crys tals in the shape of elongated plates have unit-cell dimensions a = 73 .4, b = 120.0, c = 190.1 Angstrom, orthorhombic space group P2(1)2(1)2 (1) Monoclinic prisms have unit-cell dimensions a = 67.7, b = 104.3, c = 52.8 Angstrom, beta = 105 degrees, space group P2(1). Diffraction t o slightly better than 3.0 Angstrom has been observed for both forms u sing in-house and synchrotron facilities. These crystal forms may aid the structure solution of this enzyme by presenting additional forms f or heavy-atom derivatization. These forms have multiple copies of the enzyme in the asymmetric unit and averaging methods might also be usef ul in the analysis.