ENZYMATIC PREPARATION OF BIOSURFACTANTS FROM SUGARS OR SUGAR ALCOHOLSAND FATTY-ACIDS IN ORGANIC MEDIA UNDER REDUCED PRESSURE

Citation
A. Ducret et al., ENZYMATIC PREPARATION OF BIOSURFACTANTS FROM SUGARS OR SUGAR ALCOHOLSAND FATTY-ACIDS IN ORGANIC MEDIA UNDER REDUCED PRESSURE, Biotechnology and bioengineering, 48(3), 1995, pp. 214-221
Citations number
29
Categorie Soggetti
Biothechnology & Applied Migrobiology
ISSN journal
00063592
Volume
48
Issue
3
Year of publication
1995
Pages
214 - 221
Database
ISI
SICI code
0006-3592(1995)48:3<214:EPOBFS>2.0.ZU;2-8
Abstract
Biosurfactants were prepared by enzymatic esterification of sugars and sugar alcohols in nonaqueous media. Sorbitol monooleate was produced in pure molten substrates, with reduced pressure to remove water. The results were compared to synthesis in organic solvent, with and withou t water removal, Synthesis in organic solvent with water removal, obta ined by refluxing through a desiccant under reduced pressure, proved t o be the most efficient method in terms of total yield and side-produc ts formation. This process was applied to the production of different surfactants, by changing the nature of the hydrophilic and hydrophobic moieties. Yields above 90% of monoesters were obtained after 24 h whe n the reaction was carried out in 2-methyl-2-butanol with Novozym 435 (Type B lipase from Candida antarctica) with an excess of hydroxyl don or. (C) 1995 John Wiley & Sons, Inc.