ISOZYME ANALYSIS OF 3 SPECIES OF ROEGNERIA KOCH,C. FROM CHINA

To collect and exploit Roegneria genetic resources, isozyme variation on 7 different enzymes encoded by 26 presumptive loci were analyzed in leaf extracts of R. kamoji, R, ciliaris, R. nakaii and Triticum aesti vum cv. Chinese Spring (control species) using polyacrylamide gel elec trophoresis. No isozyme polymorphism was detected within accession of the three Roegneria species, all having self-pollinating reproduction. However, extensive variations at isozyme loci were observed among acc essions of R. kamoji - even among accessions distributed at different altitudes within the same collection area. These results suggested tha t: (1) a select few individuals collected at a collection site may be enough to represent the genetic variability of that population; and (2 ) collection and maintenance of R. kamoji materials from different alt itudes may be required to increase genetic diversity. Furthermore, the banded phenotypes at EST-1 and EST-2 loci may be used as a biochemica l marker associated with a morphological character, pubescences covere d at leaf margin in R. kamoji. The banded characters at EST-1, SOD-1, SOD-2, and SOD-3a loci may be used as biochemical markers to identify the R. kamoji chromosomes carrying these loci in a T. aestivum x R, ka moji hybridization program.