HYPOCHLORITE-INDUCED DESTRUCTION OF LIPID PHASE OF HUMAN PLASMA-LIPOPROTEINS

Oxidative degradation of human lipoproteins (LP) was studied in the pr esence of exogenous sodium hypochlorite (NaOCl). Using thin-layer chro matography (TLC) and high-performance liquid chromatography (HPLC), we showed the changes in the LP lipids. We demonstrated, that the lipid component of low density lipoproteins (LDL) was more sensitive to the destruction by NaOCl, than of the very low density (VLDL) and high den sity lipoproteins (HDL). The LDL lipids of LDL were complete degraded after the addition of 1 mM NaOCl. The interaction between NaOCl and ap oproteins was non specific. These results suggest that endogenous OCl- is involved in the modification of human plasma LP in vivo.