Jm. Whalley et al., CHARACTERISTICS OF EQUINE HERPESVIRUS-1 GLYCOPROTEINS EXPRESSED IN INSECT CELLS, Veterinary microbiology, 46(1-3), 1995, pp. 193-201
A series of recombinant baculoviruses containing genes for glycoprotei
ns C, D, H and L, of equine herpesvirus 1 (EHV-1) have been constructe
d, and the EHV-1 products characterised by gel electrophoresis and imm
unoblotting. The EHV-1 glycoproteins expressed in insect cells were si
milar but not identical in apparent sizes to those expressed in EHV-1
infected mammalian cells. Each of the EHV-1 products was recognised by
convalescent equine sera, indicating that they were all targets for a
n equine immune response. Mice immunised with baculovirus-expressed EH
V-1 gD and gC acquired an enhanced ability to clear challenge EHV-1 fr
om respiratory tissues, in association with both neutralising antibody
and cell mediated immune responses.