We recorded vocalizations of megachiropteran bats, Cynopterus brachyot
is, C. horsfieldi, Megaerops ecaudatus and Macroglossus sobrinus as th
ey were held in our hands, in a hanging position or moved up and down,
simulating flight. All four species produced regular audible tone pul
ses with a frequency spectrum of less than 9 kHz (peak at 4-6 kHz). Th
e duration of a single pulse varied from 14.9 to 23.6 msec on average
among these species. The interpulse interval was between 108.3 and 127
.7 msec. In M. ecaudatus and M. sobrinus, double pulses were occasiona
lly emitted, with interpulse intervals averaging 39 msec and 31 msec,
respectively. The regular pulse emission may indicate that the bats st
udied use sounds to probe space.