EXPRESSION OF INTERCELLULAR AND VASCULAR CELL-ADHESION MOLECULES AND CLASS-II MAJOR HISTOCOMPATIBILITY ANTIGENS IN HUMAN LUNGS - LACK OF INFLUENCE BY CONDITIONS OF ORGAN PRESERVATION
S. Hasegawa et al., EXPRESSION OF INTERCELLULAR AND VASCULAR CELL-ADHESION MOLECULES AND CLASS-II MAJOR HISTOCOMPATIBILITY ANTIGENS IN HUMAN LUNGS - LACK OF INFLUENCE BY CONDITIONS OF ORGAN PRESERVATION, The Journal of heart and lung transplantation, 14(5), 1995, pp. 897-905
Background: The expression of intercellular adhesion molecule-1, vascu
lar cell adhesion molecule-1, and class II major histocompatibility co
mplex antigens was studied in control lung tissue and preserved human
donor lungs. The three controls were represented by wedge biopsy speci
mens taken from non-neoplastic lung surrounding bronchogenic carcinoma
s. Methods: Nine lungs were harvested from six brain-dead donors, flus
hed with Euro-Collins solution or low potassium-dextran-glucose soluti
on, and stored at 1 degrees or 10 degrees C. Samples of the latter org
ans were taken at the time of surgical harvest (baseline) and after 2,
12, 24, and 48 hours of preservation time. Immunostains with monoclon
al antibodies against intercellular adhesion molecule-1, vascular cell
adhesion molecule-1, and class II major histocompatibility complex mo
lecules were performed on all samples, and the relative presence of th
ese determinants was evaluated. Results: In both the controls and pres
erved lungs, intercellular adhesion molecule-1 expression was intense
in the septal capillary endothelium and alveolar pneumocytes, but esse
ntially absent in bronchial epithelium. Vascular cell adhesion molecul
e-1 was moderately to strongly labeled in the endothelia of large and
small blood vessels of all types, and it was not seen in other cell ty
pes. Class II major histocompatibility complex antigens were variably
observed in pulmonary epithelial cells, but they were not expressed by
endothelia. There appeared to be no significant difference in the imm
unohistologic density of intercellular adhesion molecule-1 or vascular
cell adhesion molecule-1 immunostaining in allografts at the specifie
d time points of preservation; this conclusion was confirmed by Wester
n blot analysis. Similar findings pertained to staining results for hu
man leukocyte DR antigens. There was likewise no significant differenc
e in the expression of the three analytes when donor lungs perfused wi
th Euro-Collins solution versus low potassium-dextran-glucose solution
were compared; this was also true of organs preserved at 1 degrees C
versus 10 degrees C. Conclusions: These results suggest that, in the i
mmediate postharvest period, modulations in the expression of intercel
lular adhesion molecule-1, vascular cell adhesion molecule-1, or class
II major histocompatibility complex antigens in pulmonary allografts
are not attributable to the influences of preservation conditions.