A circular genetic map of the bacterium Erwinia carotovora subsp. atro
septica 3-2 was constructed on the basis of the R471a plasmid and Tn5
and Tn9 using Hfr-like donors. Forty-six genes, including phytopathoge
nicity genes, were located on the basis of interrupted mating experime
nt results and analysis of coinheritance of markers on a map of 183 mi
n in length. The similarity and differences of chromosomal genetic map
s of Erwinia genus bacteria are discussed.