M. Kakeda et al., AN ESCHERICHIA-COLI CURVED DNA-BINDING PROTEIN WHOSE EXPRESSION IS AFFECTED BY THE STATIONARY-PHASE SPECIFIC SIGMA-FACTOR SIGMA(S), MGG. Molecular & general genetics, 248(5), 1995, pp. 629-634
From Escherichia coli, a DNA-binding protein that preferentially recog
nizes a curved DNA sequence was isolated and shown to correspond to on
e that has recently been reported as a binding protein for the replica
tion origin of the E. coli chromosome, named Rob. Here, a rob promoter
-lacZ transcriptional fusion was constructed on the chromosome, and us
ed to demonstrate that the expression of rob is notably enhanced at th
e onset of stationary phase in Luria-broth and also under certain grow
th conditions in a minimal medium, such as glucose- and phosphate-star
vation medium. It was further shown that this growth condition-depende
nt expression of rob is notably reduced in a null mutant for the stati
onary phase-specific sigma subunit of RNA polymerase, sigma(s), althou
gh sigma(s)-independent expression of rob was significant during the l
ogarithmic growth phase. Furthermore the rob null mutant was found to
exhibit, as compared with the wild-type, an altered profile of protein
synthesis, particularly at the very late stationary phase.