PDCSA VECTORS FOR STRICTLY REGULATED PROTEIN-SYNTHESIS DURING EARLY DEVELOPMENT OF DICTYOSTELIUM-DISCOIDEUM

Authors
FAIX J DITTRICH W PRASSLER J WESTPHAL M GERISCH G
Citation
J. Faix et al., PDCSA VECTORS FOR STRICTLY REGULATED PROTEIN-SYNTHESIS DURING EARLY DEVELOPMENT OF DICTYOSTELIUM-DISCOIDEUM, Plasmid, 34(2), 1995, pp. 148-151
Citations number
23
Categorie Soggetti
Genetics & Heredity
Journal title
Plasmid → ACNP
ISSN journal
0147619X
Volume
34
Issue
2
Year of publication
1995
Pages
148 - 151
Database
ISI
SICI code
0147-619X(1995)34:2<148:PVFSRP>2.0.ZU;2-W
Abstract
Two expression vectors have been constructed to express proteins exclu sively in developing cells of Dictyostelium discoideum. In these Esche richia coli/D. discoideum shuttle vectors, proteins are synthesized un der control of the promoter of the contact site A (csA) gene, which is efficiently suppressed during growth and becomes strongly activated d uring early development of D. discoideum. The pDcsA vectors appear to be valuable tools for the production of proteins that are not compatib le with growth of D. discoideum cells. (C) 1995 Academic Press, Inc.