COMPONENTS OF THE ADRENERGIC SIGNAL-TRANSDUCTION PATHWAY IN AMERICAN EEL AND BLACK BULLHEAD LIVERS

This study examined the epinephrine (EPI)-induced changes in second me ssengers of hepatocytes isolated from two teleost fishes, the American eel (Anguilla rostrata) and the black bullhead (Ictalurus melas). EPI al 10 mu M stimulated the production of cAMP and IP3 in both hepatocy tes, although the changes in cAMP were greater than those for IP3. Spe cific binding of H-3-prazosin by purified hepatic membranes fits in wi th the existence of alpha(1)-adrenoceprors on liver membranes from bot h species. These data support a role for both signal transduction path ways in the mode of action of EPI on fish liver cells.