NUTRITION OF TEMPE MOLDS

The nutritional requirements in vitro of Amylomyces rouxii NRRL 3160, Rhizopus arrhizus NRRL 1469, R. arrhizus NRRL, 1526, R. oligosporus NR RL 2710, R. oligosporus NRRL 5905, R. oligosporus NRRL A-10457, R. ory zae IMI 215407, R. oryzae NRRL 3563 and R. stolonifera NRRL A-2293 wer e investigated. All strains grew in glucose-ammonium-salts medium with out addition of vitamins. None utilized, as sole carbon and energy sou rce, raffinose or stachyose, the main flatulence-associate oligosaccha rides in soybean. All the strains, except Rhizopus oryzae IMI 215407, utilized one or more long chain fatty acids as sole sources of carbon and energy but only the R. arrhizus strains, R. oryzae NRRL 3563 and R , stolonifera, were able to use glycerol as sole carbon and energy sou rce. Phytic acid was used neither as a source of carbon nor of phospha te by any strain.