THE IMPORTANCE OF METHANOGENS ASSOCIATED WITH CILIATE PROTOZOA IN RUMINAL METHANE PRODUCTION IN-VITRO

Citation
Cj. Newbold et al., THE IMPORTANCE OF METHANOGENS ASSOCIATED WITH CILIATE PROTOZOA IN RUMINAL METHANE PRODUCTION IN-VITRO, Letters in applied microbiology, 21(4), 1995, pp. 230-234
Citations number
16
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
02668254
Volume
21
Issue
4
Year of publication
1995
Pages
230 - 234
Database
ISI
SICI code
0266-8254(1995)21:4<230:TIOMAW>2.0.ZU;2-7
Abstract
The importance of methanogenic bacteria associated with ciliate protoz oa was estimated either by removing protozoa from whole rumen fluid (u sing defaunated rumen fluid to correct for the effects of centrifugati on on bacteria) or by isolating the protozoa. Rumen fluid was withdraw n from sheep inoculated with either Polyplastron multivesiculatum, a c o-culture of Isotricha prostoma plus Entodinium spp. or a mixed type B fauna of Entodinium, Eudiplodinium and Epidinium spp. Methanogenesis was highest in rumen fluid containing a mixed protozoal population of the following genera: Entodinium, Eudiplodinium and Epidinium, was low er in defaunated rumen fluid and lowest in rumen fluid containing eith er I, prostoma plus Entodinium or P. multivesiculatum. Methanogenic ba cteria associated with rumen ciliates were apparently responsible for between 9 and 25% of methanogenesis in rumen fluid.