IMMUNOHISTOCHEMICAL LOCALIZATION OF NAD GLYCOHYDROLASE IN HUMAN AND RABBIT-TISSUES

NAD glycohydrolase (NADase) is present in many organisms from bacteria to mammals. In any given organism, this enzyme is ubiquitous in many tissues. However, its precise localization and its physiological signi ficance have not been defined. We have determined the distribution of NADase in normal human and rabbit tissues by immunoblotting and immuno histochemistry, using a polyclonal antibody raised in goats. Immunoblo t analyses revealed that NADase was highly expressed in the heart, lun g, stomach, and liver tissues of the rabbit. From immunohistochemical studies of NADase, high concentrations in both human and rabbit tissue s were found in hepatocytes and sinusoidal lining cells, sinus histioc ytes of the lymph node, spleen and thymus, glomerular capillary endoth elial cells of the kidney, cardiac muscle, endothelium of blood vessel s, and erythrocytes.