ISOLATION AND COMPARATIVE CHARACTERIZATION OF PEROXIDASES FROM THE FUNGUS PHELLINUS-IGNIARIUS (L, FR) QUEL-90-1 AND FROM TOBACCO

Peroxidase from the wood-degrading fungus Phellinus igniarius and the lignin-forming acidic peroxidase from tobacco Nicotiana sylvestris wer e isolated and characterized for the first time. Properties of the iso lated enzymes and their substrate specificity were studied in comparis on with the alkaline peroxidases from horseradish roots and alfalfa ce ll culture. The novel fungal peroxidase was shown to be neither lignin peroxidase nor Mn-dependent peroxidase; it belongs to a class of secr eted fungal peroxidases of the classic plant type. Similarity in the p roperties of the fungal and tobacco peroxidases is noted.