M. Herrmann et al., FAST AND HIGH-AFFINITY BINDING OF B-LYMPHOTROPIC PAPOVAVIRUS TO HUMANB-LYMPHOMA CELL-LINES, Journal of virology, 69(11), 1995, pp. 6797-6804
Binding of B-lymphotropic papovavirus (LPV) to host cells differing in
susceptibility to viral infection was determined by a newly establish
ed, direct, nonradioactive virus binding assay, which allows quantitat
ive description of the binding characteristics by receptor saturation
and Scatchard analysis, LPV binding to the highly susceptible human B-
lymphoma cell line BJA-B K88 is specific, saturable, and noncooperativ
e, Binding occurs very fast, with an association rate constant (k(1))
of 6.7 x 10(7) M(-1) s(-1), and is of high affinity, with a dissociati
on constant (K-d) of 2.9 x 10(-12) M; and the virus-receptor complex i
s stable, with a half life of 70 min, The binding affinities of recept
ors on four other highly, moderately, or weakly susceptible human B-ly
mphoma cell lines were similar, with up to twofold variation around a
mean K-d value of 3 x 10(-12) M, suggesting the presence of the same L
PV receptor on all of these cell lines, This view is further supported
by the finding that in all cases a terminal sialic acid is necessary
for LPV binding, Tunicamycin has been shown to drastically induce LPV
susceptibility and LPV binding in weakly and moderately susceptible B-
lymphoma cell lines (O, T, Keppler, M, Herrmann, M, Oppenlander, W, Me
schede, and M, Pawlita, J, Virol, 68:6933-6939, 1994), The hypothesis
that the constitutively expressed and tunicamycin-induced LPV receptor
s are identical is strengthened by our finding that both receptor type
s displayed the same high affinity. LPV susceptibility of different B-
lymphoma cell lines was correlated with receptor number but not with r
eceptor affinity, The numbers of receptors per cell on highly and mode
rately susceptible cell lines ranged from 2,000 to 400 and were direct
ly proportional to LPV susceptibility, This indicates that the number
of high-affinity receptors per cell is a key regulating factor for the
LPV host range.