[I-125] IGM (KAU) HUMAN MONOCLONAL COLD AGGLUTININ - LABELING AND STUDIES ON ITS BIOLOGICAL-ACTIVITY

Authors
VIGORITO E ROBLES A BALTER H NAPPA A GONI F
Citation
E. Vigorito et al., [I-125] IGM (KAU) HUMAN MONOCLONAL COLD AGGLUTININ - LABELING AND STUDIES ON ITS BIOLOGICAL-ACTIVITY, Applied radiation and isotopes, 46(10), 1995, pp. 975-979
Citations number
12
Categorie Soggetti
Nuclear Sciences & Tecnology","Radiology,Nuclear Medicine & Medical Imaging
Journal title
Applied radiation and isotopes
ISSN journal
09698043 → ACNP
Volume
46
Issue
10
Year of publication
1995
Pages
975 - 979
Database
ISI
SICI code
0969-8043(1995)46:10<975:[I(HMC>2.0.ZU;2-1
Abstract
In order to study the interaction between an IgM cold agglutinin and t he erythrocyte I antigen, the former antibody was labelled with I-125 using the Chloramine-T, IODOGEN and Bolton-Hunter methods. High incorp oration and adequate stability of the labelled IgM were obtained with all procedures. However, suitable biological activity was maintained o nly with the Bolton-Hunter method. Further studies suggest that tyrosi ne iodination affects antigen recognition by this IgM, whereas iodinat ion of amino groups does not. The reagent thus prepared allowed the de termination of the number of I sites per erythrocyte as well as the an tibody affinity constant.