COMPARATIVE CHARACTERIZATION OF THE FERMENTATION PATHWAY OF SACCHAROMYCES-CEREVISIAE USING BIOCHEMICAL SYSTEMS-THEORY AND METABOLIC CONTROLANALYSIS - STEADY-STATE ANALYSIS

In the preceding paper in this issue, we have shown that metabolic con trol analysis and biochemical systems theory use the same experimental information to describe a metabolic system. In this paper, we analyze the steady-state properties of this pathway by applying both methods. Our results show the correspondence of the steady-state characterizat ions and illustrate the relationships between the different nomenclatu res used. With both approaches, we identify metabolite pools that are strongly influenced by changes in enzyme concentration when cells are immobilized at pH 5.5. In the final paper of this series, which follow s, we discuss the need to assess the quality of a model and the potent ial difficulties that may arise if the steady-state characterization i s accepted without testing its quality. We then validate the different models using parameter sensitivity concepts.