STIMULATION OF CLONED HUMAN GLUCAGON-LIKE PEPTIDE-1 RECEPTOR EXPRESSED IN HEK-293 CELLS INDUCES CAMP-DEPENDENT ACTIVATION OF CALCIUM-INDUCED CALCIUM-RELEASE

Citation
J. Gromada et al., STIMULATION OF CLONED HUMAN GLUCAGON-LIKE PEPTIDE-1 RECEPTOR EXPRESSED IN HEK-293 CELLS INDUCES CAMP-DEPENDENT ACTIVATION OF CALCIUM-INDUCED CALCIUM-RELEASE, FEBS letters, 373(2), 1995, pp. 182-186
Citations number
19
Categorie Soggetti
Biophysics,Biology
Journal title
ISSN journal
00145793
Volume
373
Issue
2
Year of publication
1995
Pages
182 - 186
Database
ISI
SICI code
0014-5793(1995)373:2<182:SOCHGP>2.0.ZU;2-W
Abstract
The actions of glucagon-like peptide-1(7-36)amide (GLP-1(7-36)amide) o n cellular signalling were studied in human embryonal kidney 293 (HEK 293) cells stably transfected with the cloned human GLP-1 receptor. Th e cloned GLP-1 receptor showed a single high-affinity binding site (K- d = 0.76 nM). Binding of GLP-1(7-36)amide stimulated cAMP production i n a dose-dependent manner (EC(50) = 0.015 nM) and caused an increase i n the intracellular free Ca2+ concentration ([Ca2+](i)). The latter ef fect reflected Ca2+-induced Ca2+ release and was suppressed by ryanodi ne. We propose that the ability of GLP-1(7-36)amide to increase [Ca2+] (i) results from sensitization of the ryanodine receptors by a protein kinase A dependent mechanism.