A 2ND-GENERATION YAC CONTIG MAP OF HUMAN-CHROMOSOME-3

A map of human chromosome 3 which integrates both physical and genetic data has been developed from the fusion of two large collections of m arkers and corresponding yeast artificial chromosome (YAC) clones. The map contains 972 megabase-sized YACs identified with 593 primary mark ers, of which 162 are highly polymorphic sequence-tagged sites (STSs) and form a closely spaced genetic linkage map; the remaining markers a re hybridization-based. Chromosome 3 is now represented by 24 large YA C contigs whose order and orientation is largely known. The map genera ted by fusion of these hybridization- and STS-based datasets covers ab out 80% (over 160 megabases) of the chromosome and will provide the fo undation necessary for vapid development of a detailed genetic underst anding for this large autosome.