Fd. Guerrero, CHANGES IN HORN FLY HAEMATOBIA-IRRITANS (L) (DIPTERA, MUSCIDNE) TRANSLATABLE RNA ASSOCIATED WITH FEEDING AND DEVELOPMENTAL STAGE, The Southwestern entomologist, 20(3), 1995, pp. 273-280
Poly(A) RNA was purified from mature adult, larval and pupal stages of
the horn fly, Haematobia irritans (L.), and newly emerged adult flies
which had been fed citrated bovine blood for 0, 20 and 60 min or a bu
ffered saline solution for 20 min. These RNAs were characterized by in
vitro translation and SDS polyacrylamide gel electrophoresis, fluorog
raphy and autoradiography. Several RNA in vitro translation products w
ere detected which appear to be stage-specific while others are possib
ly regulated by gut enlargement following feeding. These putative feed
ing-induced RNAs, some of which respond within 20 min of engorging on
the first blood meal, may be transcribed from genes responding to feed
ing-induced gut distension changes.