Cl. Mills et al., DECREASED BETA-ADRENERGIC STIMULATION OF GLYCOPROTEIN-SECRETION IN CFMICE SUBMANDIBULAR GLANDS - REVERSAL BY THE METHYLXANTHINE, IBMX, Biochemical and biophysical research communications, 215(2), 1995, pp. 674-681
beta-adrenergic stimulation of glycoprotein secretion was shown to be
decreased in submandibular glands of Cystic Fibrosis (CF) mice, The de
fective response was partially restored by the methylxanthine, IBMX or
cpt-cyclic AMP. Cholinergic stimulation of pancreatic amylase secreti
on was not affected in CF mice, demonstrating that this is not a gener
alised depression of protein secretion. The data are the first to show
that the CF mouse mimics the protein secretion defect in CF human sub
mandibular cells and that the mechanism of correction of the CF defect
is via elevation of cyclic AMP. The results are therefore invaluable
towards devising a rational pharmaceutical therapy for CF patients. (C
) 1995 Academic Press, Inc.