VALIDATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR QUANTITATION OF NEOHESPERIDINE DIHYDROCHALCONE IN FOODSTUFFS

An analytical method to detect and quantitate neohesperidine dihydroch alcone in foodstuffs has been developed and validated in soft-drink ap plications. The method was shown to be sufficiently precise, accurate, selective and rugged in quantitating neohesperidine DC both at flavou ring (1-5 mg/kg) and sweetening (5-50 mg/kg) levels. Applications of t he method to determine neohesperidine DC in foodstuffs other than soft -drinks is also described.