K. Schmeer et al., CAPILLARY ELECTROCHROMATOGRAPHY ELECTROSPRAY MASS-SPECTROMETRY - A MICROANALYSIS TECHNIQUE, Analytical chemistry, 67(20), 1995, pp. 3656-3658
An electrospray mass spectrometer was coupled to an capillary electroc
hromatographic system. Peptides were analyzed in a capillary column pa
cked with 1.5-mu m reversed phase stationary phase at now rates of 1-2
mu L/min. Supplementary pressure was applied to stabilize the electro
chromatographic performance. The interface employed allowed fast and s
imple installation of the electrochromatographic system to the mass sp
ectrometer, requiring neither sheath now nor long transfer capillaries
. This CEC-MS coupling allows the routine detection of picomole quanti
ties.