CAPILLARY ELECTROPHORESIS STUDY OF THE HYDROLYSIS OF A BETA-LACTAMASEINHIBITOR

yl-1,2,3-triazol-4-yl)methylene]penem-3-carboxylic acid monohydrate (B RL 42715) is a potent beta-lactamase inhibitor. We report a study on t he hydrolysis of the four-membered lactam ring of this compound mediat ed either by Tris buffer or by Tem-2 beta-lactamase. In both studies f ree-zone capillary electrophoresis proved to be ideal in the identific ation of the products of reaction, the interpretation of the mechanism of hydrolysis, and the determination of kinetic parameters and stoich iometry.