SEPARATION MECHANISM EXPLORATION ON METAL-MBTAE-SALICYLIC ACID MIXED-LIGAND COMPLEXES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The separation and determination of platinum metal and co-existing met al complexes by reversed-phase high performance liquid chromatography (HPLC) with 2-(6-methyl-2-benzothiazolylazo)-5-diethylamino phenol (MB TAE) as a precolumn derivatizing reagent is presented. The separation mechanism of these complexes was investigated by combining spectrophot ometry with HPLC while salicylic acid was contained in the mobile phas e. The results show that most platinum metal ions, Co(II) and Cu(II) c an form ternary mixed ligand complexes with MBTAE and salicylic acid. The relationship of the retention behavior of complexes and the surfac e tension of the mobile phase (gamma), the column temperature (T), and the composition and space configuration of complexes was also investi gated. Some possible configurations of complexes are also proposed. Th ese may all be illustrated well from the viewpoint of solvophobic theo ry. These method allowed the prediction of the composition and structu re of metal complexes by utilizing HPLC.