By assaying lysozyme activity after denaturing polyacrylamide gel elec
trophoresis of commercial hen egg white lysozyme preparations, minor l
ysozymal activity was detected as an 18-kDa protein. After electrophor
etic purification for microsequencing, the N-terminus sequence of the
18-kDa lysozyme was found to be identical with mature 14.4-kDa hen egg
white lysozyme. The 18-kDa hen egg white lysozyme was judged to be gl
ycosylated based on a 3.6-kDa decrease in molecular mass after N-glyco
sidase F treatment, binding to concanavalin A - Sepharose, and stainin
g with periodate - Schiff's reagent. The minor form corresponded to ab
out 0.3% of lyzozyme molecules.