Mm. Welle et al., DEMONSTRATION OF TRYPTASE IN BOVINE CUTANEOUS AND TUMOR MAST-CELLS, The Journal of histochemistry and cytochemistry, 43(11), 1995, pp. 1139-1144
We examined three tissue samples from each of four cows with non-lesio
nal skin, tissue samples from a cow with multiple cutaneous mast cell
tumors, and samples from another cow in which mast cells were infiltra
ting multiple lymphosarcomas of the skin, for the presence of tryptase
and chymase by enzyme cytochemical and immunohistological methods, Th
e enzyme activities of tryptase and chymase were tested using bobenzox
y-glycilglycil-L-arginine-2-naphthyl-amide (Z-Gly-Gly-Arg-NA) and naph
thol-AS-D-chloroacetate (N-AS-D-CA) as substrates, respectively, Trypt
ase reactivity could be demonstrated in frozen and Carnoy-fixed paraff
in sections, Chymase reactivity was seen in neither frozen nor paraffi
n sections of formalin- or Carnoy-fixed skin tissues, Antibody linkage
with a polyclonal rabbit anti-human skin trypase antibody was highly
specific in bovine normal cutaneous, infiltrating, and tumor mast cell
s, More than 90% of the tumor mast cells were distinctly trypase-posit
ive, With alcian blue, only slightly more than 10% of the mast cells s
tained dearly positive and with methylene blue hardly any staining of
mast cell granules could be demonstrated, No antibody labeling of mast
cell granules in any of the tissue sections was detected by the use o
f rabbit anti-dog chymase antiserum, These results indicate that there
is a striking antigenic similarity of bovine trypase to its canine an
d human equivalents, The demonstration of trypase is an important tool
in confirming the diagnosis of undifferentiated mast cell tumors, In
contrast to other species, chymase appears to be completely absent in
bovine skin mast cells.