USE OF DOUBLE-LABELING AND PHOTO CD FOR MORPHOMETRIC ANALYSIS OF INJURED SKELETAL-MUSCLE

We used computer-assisted analysis of myofiber cross-sectional areas t o measure skeletal muscle responses to injury and disease. We develope d a simple, inexpensive method for measuring myofiber size in human mu scle samples using Kodak photo compact discs (CDs) as the image source . The photo CD serves as a permanent image storage medium and provides a high-resolution image that can be used to detect small myofibers. T he use of double labeling for dystrophin and desmin allowed positive i dentification of both degenerating and regenerating fibers in a single biopsy specimen.