QUANTIFICATION OF GLYCEOLLINS IN NON-ELICITED SEEDLINGS OF GLYCINE-MAX BY GAS CHROMATOGRAPHY MASS SPECTROMETRY

A sensitive gas chromatography-mass spectrometry method is described f or detection and quantification of small amounts of phytoalexins, espe cially glyceollins. This method allowed identification of canescacarpi n (glyceollin V) besides appreciable amounts of glyceollin isomers I-I II and glyceofuran in unchallenged parts of soybean seedlings. Based o n this quantification method the glyceollin content of soybean roots, either untreated, wounded or incubated with a glucan elicitor from Phy tophthora sojae, was compared. The proportions of glyceollin isomers i n the mixture of compounds were determined in different soybean tissue s. Single-ion monitoring revealed the presence of four additional glyc eollin isomers with benzofuranoid structure.