DISTANCE BETWEEN ALLELES AS A DETERMINANT OF LINKAGE IN NATURAL TRANSFORMATION OF ACINETOBACTER-CALCOACETICUS

Authors
KLOOS DU DIMARCO AA ELSEMORE DA TIMMIS KN ORNSTON LN
Citation
Du. Kloos et al., DISTANCE BETWEEN ALLELES AS A DETERMINANT OF LINKAGE IN NATURAL TRANSFORMATION OF ACINETOBACTER-CALCOACETICUS, Journal of bacteriology, 177(20), 1995, pp. 6015-6017
Citations number
18
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
177
Issue
20
Year of publication
1995
Pages
6015 - 6017
Database
ISI
SICI code
0021-9193(1995)177:20<6015:DBAAAD>2.0.ZU;2-E
Abstract
Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 k b, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipie nt alleles was reduced from 9.2 to 5.1 kb. Ligation of unlinked chromo somal DNA fragments allowed them to be linked genetically through natu ral transformation.