Pj. Baxter et al., MULTIVARIATE APPROACH FOR THE SIMULTANEOUS DETERMINATION OF TOTAL BIOMASS AND GLUCOSE FROM A YEAST FERMENTATION BY SEQUENTIAL INJECTION-ANALYSIS, Chemia Analityczna, 40(4), 1995, pp. 455-471
A simultaneous determination of total biomass and glucose was performe
d using a simple sequential injection system with the full range of vi
sible absorbance spectrum al the analytical signal. The assays were pe
rformed on-line with no pre-treatment of the sample. fn the past, meas
urement on unfiltered bioprocess samples have been avoided due to anti
cipated problems such as cell aggregation, clogging of the flow system
, cell adhesion to tubing walls and cross contamination. The sample wa
s diluted, mixed with the glucose reagent in a stirred cell and the re
sulting signal was collected as a function of time. Calibration models
were determined using linear partial least square's regression and te
sted by a cross-validation. The average difference between the model's
prediction of total biomass and the dry weight determination was 5 %.
The glucose model lacked quantitative predictive ability but could id
entify pulse spikes in the Fermentation broth. The sampling frequency
was 7 samples/h.