GENETICS AND MAPPING OF NEW ISOZYME LOCI IN VICIA-FABA L USING TRISOMICS

Polymorphism in ten enzyme systems (AGO, ACP, AAT, EST, FK, ME, NAG, P RX, 6PGD, and SOD) in Vicia faba L. was analyzed, revealing 13 loci, s ix of which have not been reported before. Inheritance, genetics, poss ible location, and linkage analysis were studied in 13 different F-2 p opulations trisomic for four of the six chromosomes (nos. 3, 4, 5, and 6) of the species. Each of these loci exhibited typical Mendelian inh eritance except for those involved in the trisomic chromosome. Five lo ci have been assigned to a specific chromosome: Est-2 to chromosome 3, Fk-2 to chromosome 4, Prx-1 to chromosome 5, and Sod-1 and Pgd-p to c hromosome 6. Nag-1 and Pgd-c displayed a linkage of 22.8 cM indicating a clear homology with chromosome 5 of garden pea on which both marker s are syntenic.