INTERSPECIFIC TRANSFER OF ISOLATED PLANT-MITOCHONDRIA BY MICROINJECTION

Citation
Ha. Verhoeven et al., INTERSPECIFIC TRANSFER OF ISOLATED PLANT-MITOCHONDRIA BY MICROINJECTION, Plant cell reports, 14(12), 1995, pp. 781-785
Citations number
34
Categorie Soggetti
Plant Sciences
Journal title
ISSN journal
07217714
Volume
14
Issue
12
Year of publication
1995
Pages
781 - 785
Database
ISI
SICI code
0721-7714(1995)14:12<781:ITOIPB>2.0.ZU;2-8
Abstract
Data on isolation, purification and transfer of mitochondria from a cy toplasmic male sterile line of the ''Ogura'' type of Raphanus sativus to a male fertile line of Brassica napus are reported. Microinjection has been used for the transfer of the donor mitochondria to the recipi ent protoplasts. The injected protoplasts were identified and followed individually throughout their development using a computerized micros cope stage which greatly enhanced the number of injections (five-fold) . The transferred donor mitochondria were stably maintained during sev eral successive cell divisions, revealing that they were viable and fu nctional. Several calluses were obtained from injected protoplasts wit hout using any selection pressure. Restriction fragment length analysi s of seventeen calluses, using mitochondrial DNA probes, indicated tha t three contained the donor ''Ogura'' type mitochondria. No recombinan t types of mitochondria have been observed. Flow cytometric and karyot ype analyses of the calluses revealed the presence of similar amount o f DNA and chromosome number as those of the recipient plants of B.napu s. The application of microinjection for the manipulation of cytoplasm ic composition is discussed.