H. Hintze et al., STEREOMICROSCOPY, FILM RADIOGRAPHY, MICRORADIOGRAPHY AND NAKED-EYE INSPECTION OF TOOTH SECTIONS AS VALIDATION FOR OCCLUSAL CARIES DIAGNOSIS, Caries research, 29(5), 1995, pp. 359-363
In establishing a trustworthy validation for occlusal caries lesions,
various methods have been used after sectioning teeth. The aim of the
present study was to compare the accuracy of four such methods: stereo
microscopy (SM), film radiography (FR), microradiography (MR) and nake
d-eye inspection (NEI) for the detection of caries in occlusal tooth s
urfaces. Further, the interobserver agreement between three observers'
registrations of occlusal caries by these methods was evaluated. The
material consisted of 18 unerupted third molars known to be sound with
respect to caries, as they were embedded in bone prior to removal, an
d 20 erupted third molars with an unknown 'true state of disease'. The
teeth were serially sectioned, coded and examined blindly for occlusa
l caries by three independent observers by the four validation methods
under study. SM was demonstrated to be the only method by which all o
bservers correctly identified all 18 unerupted teeth as sound, resulti
ng in a specificity of 1.00. By the other three methods, 1 or more of
the unerupted teeth were falsely classified as carious. In the erupted
teeth, 73, 58, 52 and 32%, respectively, of the teeth were determined
carious by SM, MR, NEI and FR. As it was possible by SM to detect car
ies in nearly three quarters of the erupted teeth and at the same time
to identify all unerupted teeth as sound, this method seemed to be th
e most trustworthy of the validation methods under study.