EXPRESSION OF ARGININE DECARBOXYLASE IS INDUCED DURING EARLY FRUIT-DEVELOPMENT AND IN YOUNG TISSUES OF PISUM-SATIVUM (L)

A cDNA coding for arginine decarboxylase (ADC, EC 4.1.1.19)has been is olated from a cDNA library of parthenocarpic young fruits of Pisum Sat ivum (L.). The deduced aminoacid sequence is 74%, 46% and 35% identica l to ADCs from tomato, oat and Escherichia coli, respectively. When th e pea ADC cDNA was put under the control of the galactose inducible ye ast promoter CYC1-GAL10 and introduced into Saccharomyces cerevisiae, it conferred galactose-regulated expression of the,ADC activity. The A DC activity expressed in S. cerevisiae was inhibited 99% by alpha-DL-d ifluoromethylarginine (DFMA), a specific inhibitor of ADC activity. No activity was detected in the untransformed S. cerevisiae, nor when it was transformed with an antisense ADC construct. This provides direct evidence that the ADC cDNA from pea encoded a functional, specific AD C activity and that S. cerevisiae is able to process correctly the pro tein. In the pea plant, gene expression of the;ADC is high in young de veloping tissues like shoot tips, young leaflets and flower buds. Full y expanded leaflets and roots have much lower, but still detectable, l evels of the ADC transcript. In the ovary and fruit, they are developm entally regulated, showing high levels of expression during the early stages of fruit growth, which in pea is mainly due to cell expansion. The observed changes in the steady-state levels of ADC mRNA alone, how ever, cannot account for the differences in ADC activity suggesting th at other regulatory mechanisms must be acting.