Eighteen polymerase chain reaction (PCR) products of the partial seque
nce of the Adh (alcohol dehydrogenase) gene from 10 Brachyscome specie
s were sequenced and compared. These products contained the 5' three f
ourths of exon 4 and whole sequences of intron 3. They varied extensiv
ely in length due to the differences in length of intron 3. A total of
10 long insertions were flanked by direct repeats of 5 to 12 bp seque
nces, indicating inserted elements. These inserted elements were class
ified into the following five categories based on nucleotide sequence
characteristics and length; (1) a region homologous to that of 5S RNA
genes (5S DNA), (2) A-rich structure at the 3' end-like short interspe
rsed elements (SINEs) in animals, (3) a sequence of 280 bp with no cha
racteristic features, (4) a sequence of 125 bp with no characteristic
features, (5) termini of 11 bp inverted repeats flanked by 5 bp sequen
ce of direct repeats characteristics of a transposon.