CHARACTERIZATION OF THE STRUCTURE AND DETERMINATION OF MESSENGER-RNA LEVELS OF THE PHENYLALANINE AMMONIA-LYASE GENE FAMILY FROM POPULUS-KITAKAMIENSIS

Authors
OSAKABE Y OSAKABE K KAWAI S KATAYAMA Y MOROHOSHI N
Citation
Y. Osakabe et al., CHARACTERIZATION OF THE STRUCTURE AND DETERMINATION OF MESSENGER-RNA LEVELS OF THE PHENYLALANINE AMMONIA-LYASE GENE FAMILY FROM POPULUS-KITAKAMIENSIS, Plant molecular biology, 28(6), 1995, pp. 1133-1141
Citations number
20
Categorie Soggetti
Plant Sciences",Biology
Journal title
Plant molecular biology → ACNP
ISSN journal
01674412
Volume
28
Issue
6
Year of publication
1995
Pages
1133 - 1141
Database
ISI
SICI code
0167-4412(1995)28:6<1133:COTSAD>2.0.ZU;2-Y
Abstract
We isolated two new PAL genes, palg2b and palg4, from Populus kitakami ensis. palg2a and palg2b are clustered and palg2b encodes a polypeptid e of 710 amino acids. The nucleotide sequence in the coding region of palg2b was 94.6% identical to that of palg2a. The promoter regions of palg1, palg2a and palg2b have several elements conserved among many ph enylpropanoid biosynthetic genes. We measured the mRNA levels of the f our PAL genes by S1 mapping using total RNA from stem tissues developi ng secondary xylem. Results showed that the transcript level of palg2b was higher than that of the other PAL genes.