Fr. Mattig et al., LIGHT AND ELECTRON-MICROSCOPY STUDY OF FIRST GENERATION DEVELOPMENT OF EIMERIA-PAPILLATA (APICOMPLEXA, EIMERIIDAE) IN POLARIZED MDCK-CELLS IN-VITRO, Archiv fur Protistenkunde, 146(1), 1995, pp. 59-67
The early development of Eimeria papillata (Apicomplexa) was studied i
n vitro using light and electron microscopy. The first developmental s
tages observed were elongated, sporozoite-shaped schizonts with two nu
clei and two refractile bodies which appeared 17 hours p.i.; the schiz
onts retained this shape until the 8-nuclei-stage. At 23 hours p.i. th
ey had changed into a spherical shape, the refractile bodies had multi
plied and the development of merozoites was observed. Each merozoite o
f the first generation contained two refractile bodies. An improved me
thod for in vitro cultivation of Eimeria in polarized host cells and s
ubsequent fine structural investigation is described.