V. Delorme et al., USE OF IMMUNOCHEMICAL AND SSCP ANALYSES TO TEST HOMOZYGOSITY AT THE S-LOCUS OF BRASSICA-OLERACEA GENOTYPES, Molecular breeding, 1(3), 1995, pp. 237-244
The self-incompatibility reaction of cruciferous plants prevents self-
fertilization and has been shown to be controlled by at least two gene
s situated at a single multiallelic locus, the S locus. One of these t
wo genes, the S locus glycoprotein (SLG) gene, encodes an abundant gly
coprotein secreted to the cell wall of stigma papillae. Identification
of tho se S alleles present at the S locus is of prime interest when
studying the self-incompatibility response and can be achieved by iden
tifying the SLG of the stigma. Here, we show that using anti-SLG antib
odies in an immunochemical analysis, combined with a SSCP (single-stra
nd conformation polymorphism) approach to characterize the correspondi
ng stigma-specific, SLG mRNA, allowed the identification of plants het
erogeneous at the S locus among populations of plants that were though
t to be homozygous for known SLG alleles. This analysis stresses the i
mportance of testing the homozygosity at the S locus of lines consider
ed inbred for a known S allele as mix-up of seeds may occur during the
breeding programme.