ENZYME-IMMUNOASSAY OF ZEINS FROM SEEDS OF NORMAL AND MUTANT (OPAQUE-2) CORN

Authors
SHUPRANOVA LV ZHMAREVA EN EGGI EE SIDOROVA VV VINNICHENKO AN GAVRILYUK IP KONAREV VG
Citation
Lv. Shupranova et al., ENZYME-IMMUNOASSAY OF ZEINS FROM SEEDS OF NORMAL AND MUTANT (OPAQUE-2) CORN, Biochemistry, 60(8), 1995, pp. 959-962
Citations number
14
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
60
Issue
8
Year of publication
1995
Pages
959 - 962
Database
ISI
SICI code
0006-2979(1995)60:8<959:EOZFSO>2.0.ZU;2-W
Abstract
alpha-Zein polypeptides have been isolated from total zeins of normal and mutant, at the Opaque-2 gene, A-204 corn seeds by semipreparative isoelectric focusing in polyacrylamide gel in the pH range of 5-8. Hig hly active antisera against seven isolated a-zein polypeptides have be en raised in rabbits, and extensive cross-reaction between the polypep tides has been demonstrated by the enzyme immunoassay technique. The a ntisera were found to react differently with the total zeins of normal and mutant A-204 corn.