HEAT-SHOCK AND STRESS-RESPONSE OF THE PARASITIC NEMATODE HAEMONCHUS-CONTORTUS

Before and after a stress treatment, larval stages and adult worms of Haemonchus contortus were tested for the induction and expression of h eat-shock proteins (HSPs) using immunoblot analysis with HSP70- and HS P65-specific monoclonal antibodies. Stress treatment or heat shock did not alter the signals obtained with these antibodies, but the amount of HSP70 differed between the successive stages. In addition, the diff erent stages were metabolically labeled with [S-35]-methionine during a temperature-shock or chemical treatment and proteins were analyzed b y sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) . The shocks resulted in an altered protein pattern. New was the de no vo expression of a 20-kDa protein of adult worms after anthelmintic tr eatment.