CEN14 SEQUENCES CAUSE SLOWER PROLIFERATION, REDUCED CELL-SIZE AND ASPOROGENY IN SACCHAROMYCES-CEREVISIAE

The introduction of CEN14-based plasmids into haploid strains of Sacch aromyces cerevisiae resulted in reduced proliferation rates and signif icantly smaller (20%) cell size than in untransformed control cells. T his could be useful to those yeast biotechnology processes that requir e high levels of gene expression but little or no yeast growth and pro liferation. In diploids similar plasmids caused asporogeny, which was possibly a consequence of the reduced cell size.