NUTRIENT-ENHANCED SURVIVAL OF AND PHENANTHRENE MINERALIZATION BY ALGINATE-ENCAPSULATED AND FREE PSEUDOMONAS SP UG14LR CELLS IN CREOSOTE-CONTAMINATED SOIL SLURRIES

Authors
WEIR SC DUPUIS SP PROVIDENTI MA LEE H TREVORS JT
Citation
Sc. Weir et al., NUTRIENT-ENHANCED SURVIVAL OF AND PHENANTHRENE MINERALIZATION BY ALGINATE-ENCAPSULATED AND FREE PSEUDOMONAS SP UG14LR CELLS IN CREOSOTE-CONTAMINATED SOIL SLURRIES, Applied microbiology and biotechnology, 43(5), 1995, pp. 946-951
Citations number
22
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
43
Issue
5
Year of publication
1995
Pages
946 - 951
Database
ISI
SICI code
0175-7598(1995)43:5<946:NSOAPM>2.0.ZU;2-Z
Abstract
The effects of nutrient amendment and alginate encapsulation on surviv al of and phenanthrene mineralization by the bioluminescent Pseudomona s sp. UG14Lr in creosote-contaminated soil slurries were examined. UG1 4Lr was inoculated into creosote-contaminated soil slurries either as a free cell suspension or encapsulated in alginate beads prepared with montmorillonite clay and skim milk. Additional treatments were free-c ell-inoculated slurries amended with sterile alginate beads, free-cell -inoculated and uninoculated slurries amended with skim milk only, and uninoculated, unamended slurries. Mineralization was determined by me asuring (CO2)-C-14 released from radiolabelled phenanthrene. Survival was measured by selective plating and bioluminescence. Inclusion of sk im milk was found to enhance both survival of and phenanthrene mineral ization by free and encapsulated UG14Lr cells.