STRUCTURE ELUCIDATION OF YERSINIABACTIN, A SIDEROPHORE FROM HIGHLY VIRULENT YERSINIA STRAINS

From cultures of Yersinia enterocolitica H1852, an iron-complexing and iron-transporting compound named yersiniabactin was isolated. The str ucture of the siderophore was determined by a variety of spectroscopic methods, including 2D NMR experiments on the metal-free ligand as wel l as its gallium complex. The metal-free Ligand, derivatives, as well as iron and gallium complexes were examined by high-resolution FAB-MS, API-MS, API-MS/MS and GC-MS. The novel siderophore contains a benzene and a thiazolidine ring, as well as two thiazoline rings (Figure 1). Its stereochemistry is noteworthy for the presence of five chiral cent ers, one of which is considerably epimerized. The compound forms stabl e complexes with trivalent cations such as ferric iron and gallium.