REDUCTION OF PATULIN IN APPLE JUICE SAMPLES - INFLUENCE OF INITIAL PROCESSING

Patulin is a secondary metabolite produced by a wide range of fungi in cluding Penicillium expansum, a common contaminant of apples. Patulin is reputed to be a mutagen and recommendations have been made that lev els should not exceed 50 ng/g in apple juice intended for human consum ption. The production of apple juice requires the use of ripe fruit, w hich may be pre-disposed to fungal contamination with P. expansum and concurrent contamination with patulin. During a study using selected o ver-ripe fruit, triplicate samples of apples were withdrawn at several points prior to maceration. The mean patulin level in the non-process ed fruit was found to be 920 ng/g, but this level dropped to 190 ng/g following an initial water treatment step. Analyses of the wash water showed that appreciable levels had been transferred from the solid to the aqueous phase. Additional removal, by hand, of rotten and damaged fruit prior to further processing, significantly reduced the mean patu lin level in the juice to 55 ng/g. High patulin levels were recorded i n the rotten fractions (mean = 2335 ng/g). Mycological analyses tended to support the chemical data, in that removal of the rotten fruit sig nificantly reduced the total fungal counts in the juice samples.