T. Soto et al., NITROGEN-SOURCE-INDUCED ACTIVATION OF NEUTRAL TREHALASE IN SCHIZOSACCHAROMYCES-POMBE AND PACHYSOLEN-TANNOPHILUS - ROLE OF CAMP AS 2ND-MESSENGER, FEMS microbiology letters, 132(3), 1995, pp. 229-232
Resting cells of the fission yeast Schizosaccharomyces pombe, suspende
d in buffer with glucose. responded to the addition of asparagine by i
ncreasing trehalase activity. This response was preceded by a peak in
cAMP concentration. The addition of the nitrogen source to resting cel
ls, devoid of the catalytic subunit of cAMP-dependent protein kinase,
produced the transient increase in cAMP but did not promote any change
in trehalase activity. In the budding yeast Pachysolen tannophilus, t
he activation of trehalase by nitrogen source was also accompanied by
a sharp peak in cAMP. These results suggest that in the two yeasts cAM
P acts as a second messenger in the transduction of the nitrogen-sourc
e-induced signal causing the activation of trehalase.