GENOME MAP OF CAMPYLOBACTER-FETUS SUBSP FETUS ATCC-27374

A physical map of the chromosome of Campylobacter fetus subsp. fetus w as constructed by using pulsed-field gel electrophoresis of restrictio n fragments generated by SalI, SmaI and NotI. Digestion of the type st rain ATCC 27374 with these restriction endonucleases resulted in gener ating 4-14 fragments. The order of the fragments was deduced from hybr idization of these restriction fragments to Southern blots of pulsed-f ield gel electrophoresis gels generated by the other two enzymes. The estimated genome size was 1160 kb. The position of several homologous and heterologous genes was determined on the circular map, These inclu ded the 2.8-kb sapA gene, encoding the 97-kDa surface array protein. T hree copies of ribosomal RNA genes for which the 16S, 23S and 5S rRNA appeared to be located in close proximity in each of the three regions . The RNA polymerase genes rpoA, rpoB, and rpoD were mapped and appear ed to be situated close together in one region, The flagellin genes (f laAB) of C. jejuni and the gyrase genes gyrA acid gyrB of C. perfringe ns and Bacillus subtilis , respectively, were used to identify the loc ations of flaAB, the gyrA and the gyrB genes on the ATCC 27374 chromos ome.