LOCAL COMPARISON OF THE GENOMES OF MYCOBACTERIUM-TUBERCULOSIS AND MYCOBACTERIUM-LEPRAE USING THE POLYMERASE CHAIN-REACTION

To facilitate comparison of the genome maps of Mycobacterium tuberculo sis and M. leprae, sequence data from the M. leprae sequencing project were used to design primers suitable for the amplification of short s egments from conserved genes by the polymerase chain reaction. In most cases, both organisms yielded products of identical size that were th en used as probes for a comparative genomic walk. The hybridization da ta often, but not always, revealed a similar organization of various r egions of both genomes. This approach should be useful for systematic comparisons of (myco)bacterial genomes.